If your IACUC protocol is classified as pain category D and/or E, you'll need to include an alternatives search, which involves:
Does your protocol include more than one animal species in pain categories D or E? If so, you need to conduct an alternatives search for each species and procedure in your protocol.
More information in the Alternatives Search requirements can be found on Cornell's IACUC website.
The Animal Welfare Act requires that minimum standards of care and treatment be provided for certain animals bred for commercial sale, used in research, transported commercially, or exhibited to the public.
Researchers submitting an IACUC protocol that is classified at pain category D or E are required to do an alternatives search. This is one part of the IACUC protocol that ensures that pain and distress in animal research subjects are minimized.